Legionella Biofilm Test Kit
The Biofilm Legionella Test™, is a breakthrough technology from hydrosense® for the rapid detection of Legionella bacteria in water – delivering test results in minutes instead of days. View our instruction video or Download data sheet (pdf)
The test kit contains all the items required to perform 5 tests – including swabs, recovery buffer, and re-suspension tubes.
- Pinpoint the contamination in a system to allow targeted cleaning.
- Demonstrate cleanliness of an area e.g. after cleaning cooling tower pack.
- A clean water sample does not equate to a clean system: proliferation of Legionella is greater in a biofilm than in water.
- Highly selective – can detect Legionella even in samples with very high background flora.
- Simple to use and fast.
- Results are easy to read and obtained within 25 minutes.
- The test allows immediate understanding and response to contaminated systems.
- Use can reduce risk of outbreaks and litigation.
- Regular use will increase confidence in your Legionella control.
The hydrosense® Legionella Field Test uses an immunochromatographic assay, to detect the presence of cell surface antigens from Legionella bacteria in a sample. The presence of antigen causes the "Test Line" to turn red in colour. A "Control Line"is included which should always turn red if the test has been performed correctly.
Product details
| Bacteria detected | Legionella pneumophila serogroup 1 |
| Detection method | Lateral flow immunochromatographic assay |
| Lower limit of detection | Up to 200 CFU per area swabbed |
| Specificity | Tested against a wide range of waterborne bacteria, including Acinetobacter calcoaceticus, Aeromonas hydrophila subsp. hydrophila, Bacillus subtilis, Burkholderia cepacia, Citrobacter freudii, Citrobacter koseri, Escherichia coli, Enterobacter cloacae, Klebsiella oxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Ralstonia pickettii, Raoultella terrigena, Streptococcus pyrogenes, Yersinia ruckeri. No cross reactions observed at concentrations <= 10^9 CFU/L. Staphylococcus aureus has been observed to cross react with the test at concentrations > 10^8 CFU/L. Legionella pneumophila serogroups 4 & 7 cross react with the test at concentrations >10^9 CFU/sample. The test does not react with any other L. pneumophila serogroups or L. species tested. |
| Test volume | 100 μL Exact volume disposable pipette provided |
| Suggested sample volume | 10 cm2 |
| Biocides | The product has been tested with a range of common biocides and biodispersants used in Legionella control, at their normal maximum operating concentration. Active ingredients tested include: glutaraldehyde, didecyl-dimethyl-ammonium chloride, isolthazoles, dibromonitrilopropionamide, ionic and non-ionic surfactants. The test gives a false positive if used with polymeric biguanide and THPS. |
| Operator time | 1-2 minutes |
| Development time | 25 minutes |
| Shelf life | 18 months from manufacture when stored at room temperature (<30°C) in original packaging |