Legionella Field Test Kit

Field Test Instruction Diagram
Field test kit instruction diagram

The Legionella Field Test™ is a breakthrough technology from hydrosense™ for the rapid detection of Legionella bacteria in water – delivering test results in minutes instead of days. View our instruction video or Download data sheet (pdf)

The test kit contains everything required to perform 10 tests – including sample collection containers and disposable pipettes.

  • These tests are optimised for use with hot and cold water systems, whirlpool spas, showers, cooling towers and industrial water systems.
  • The test is simple to use and fast, with no interpretation required.
  • Results are easy to read and obtained within 25 minutes.
  • There are no chemicals to measure or handle.
  • The test allows immediate understanding and response to the water conditions.
  • Use can reduce risk of outbreaks and litigation.
  • Regular use will increase confidence in your Legionella control.

The hydrosense™ Legionella Field Test uses an immunochromatographic assay, to detect the presence of cell surface antigens from Legionella bacteria in a sample. The presence of antigen causes the "Test Line" to turn red in colour. A "Control Line" is included which should always turn red if the test has been performed correctly.

Alternative versions of the test for specialist applications are also available

Product details

Bacteria detected Legionella pneumophila serogroup 1
Detection method Lateral flow immunochromatographic assay
Lower limit of detection Approx. 10^5 CFU/L (100 CFU/mL)
Specificity Tested against a wide range of waterborne bacteria, including Acinetobacter calcoaceticus, Aeromonas hydrophila subsp. hydrophila, Bacillus subtilis, Burkholderia cepacia, Citrobacter freudii, Citrobacter koseri, Escherichia coli, Enterobacter cloacae, Klebsiella oxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Ralstonia pickettii, Raoultella terrigena, Streptococcus pyrogenes, Yersinia ruckeri. No cross reactions observed at concentrations <= 10^9 CFU/L. Staphylococcus aureus has been observed to cross react with the test at concentrations > 10^8 CFU/L. Legionella pneumophila serogroups 4 & 7 cross react with the test at concentrations > 6x10^9 CFU/L. The test does not react with any other L. pneumophila serogroups or L. species tested.
Test volume 100 μL Exact volume disposable pipette provided
Sample parameters pH range 5-10
Sample temperature range 15-45 °C (59-113 F)
Environmental operating range 15-40 °C (59-104 F)
Free chlorine concentration < 5ppm
Total Alkanlinity <1000 ppm
Total Hardnes
Biocides The product has been tested with a range of common biocides and
biodispersants used in Legionella control, at their normal maximum
operating concentration. Active ingredients tested include:
glutaraldehyde, didecyl-dimethyl-ammonium chloride, isolthazoles,
dibromonitrilopropionamide, ionic and non-ionic surfactants.
The test gives a false positive if used with polymeric biguanide or
THPS.
Operator time <2 minutes
Development time 25 minutes
Shelf life 18 months from manufacture when stored at room temperature
(<30°C) in original packaging

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